Study on Carcinoma Stomach with Special Emphasis on the Significance of Peritoneal Wash Cytology during Laproscopy and Laparotomy in unseen Metastasis

BACKGROUND: Carcinoma stomach is one of the leading causes of cancer related deaths. The potentially curative treatment for gastric adenocarcinoma is a complete margin negative (R0) resection. Hence, surgery forms the mainstay of treatment of carcinoma stomach. The commonest pattern of gastric cancer relapse is peritoneal metastasis which is the foremost cause of death even after curative resection is done. Studies have shown that despite radiological findings ruled out metastasis, staging laparoscopy contributes substantially in upstaging the disease. Also positive peritoneal wash cytology during laparoscopy or laparotomy & proceed indicates peritoneal micromets and patients with positive peritoneal wash cytology has been regarded as stage IV disease and should be treated with palliative intent. OBJECTIVES: 1. To study the Age & Sex incidence of CA Stomach. 2. To study the presenting complaints and mode of presentation of CA stomach. 3. To categorize the anatomical site of tumor. 4. To classify the patients into curative or palliative group. 5. To study the significance of peritoneal lavage cytology during laparoscopy/laparotomy procedure. METHODS: The study was conducted on 22 patients diagnosed with adenocarcinoma of the stomach who were admitted in Government Stanley Medical College between June 2015 to September 2016. Patients admitted with gastric malignancy who satisfied the inclusion and exclusion criteria were included in the study. Upper GI scopy and CECT Abdomen were done as a routine to confirm the disease and to rule out metastasis respectively. Patients without obvious metastasis in CECT Abdomen were subjected to either staging laparoscopy or laparotomy and proceed. During diagnostic laparoscopy, patients with obvious peritoneal and liver mets were noted and analyzed. These patients were excluded from taking peritoneal wash cytology. Remaining patients with no evidence of mets (during CECT and Laparoscopy) were subjected to peritoneal wash cytology and their results were noted and analysed. RESULTS: Out of 22 patients studied, 4 were women (18%) and remaining18 were men (82%). Highest number of patients, 11 cases (50%) found to be in the age group of 40 to 60 years, 5 cases (22%) found to be in the age group of 60 years. Out of 11 patients with Metastatic CA, 9 patients were greater 50 years. Out of 10 patients with Locally Advanced CA, 7 patients were less than 50 years. Most common presenting complaints are Abdomen Pain (63%), Vomiting (63%) and LOA & LOW (77%). Out of 22 patients studied, 9 cases are smoker and alcoholic and remaining 13 cases were nonsmoker and non alcoholic. During general examination of patients included in my study, 10 cases were found to be anaemic. Out of 10 patients who are Anaemic, 7 patients were Metastatic and 3 patients were Locally Advanced. Out of 22 patients, 9 cases were presented with Gastric outlet obstruction, 7 cases had Abdominal pain, 5 case shade pigastric mass for evaluation and only1casewith UGI Bleed. The most common blood group in my study were O +ve and A+ve (7 cases each). During Upper GI Scopy, the most common site of growth were located in Antro pylorus of stomach (16 cases) percentage being 72%. In CECT (Abdomen and pelvis) of study patients, 8 patients were metastatic. The remaining14 patients subjected to D-lap / Laparotomy, 2 cases were found to be metastatic (peritonealmets) and 12 cases were subjected to peritoneal wash cytology out of which1case (8.33%) was found to be positive for peritoneal cytology. CONCLUSION: Peritoneal Wash cytology during Laparoscopy/Laparotomy though many studies have shown to Upstage the disease process from locally Advanced to Metastatic. Moreover, Positive cytologyrate 4. 4–11% reported in literature which reflects the heterogeneity of patient cohorts with variable disease severity, Experience of Pathology, Duration of sample retrieval to Sample Analysis & Differences in diagnostic criteria. In my study, there were only 1 positive cytology patients out of 12 patients (D-Lapnegative) examined. Positive cytologyrate being 8.33% Hence, institutional based study should be done to establish the significance of Peritoneal Wash cytology in CA Stomach

Factors regulating the transcription of eukaryotic protein coding genes and their mechanism of action- a review

Protein factors play a crucial role in establishing gene-specific and cell-specific regulation of the process of transcription. These include general transcription factors which recognize TATA and CCAAT boxes and which form components of the RNA polymerase II system. Specific transcription factors interact with characteristic promoter elements of individual genes. Some of the examples are SP1, glucocorticoid receptor, GCN4, GAL4 and many others. Transcription factors have a DNA binding domain demarcated from the transcription activation domain. Some factors may have an additional ligand (small molecule) binding domain. Typical structural features such as helix-turn-helix motif, zinc finger and leucine zipper have been recognized in the DNA binding domain of the transcription factors. The acidic domain of the protein factors is involved in the transcription activation process. It appears that activation is the result of the combined action of several regulatory proteins binding at different regions of the promoter. Interaction between proteins bound to DNA but seperated by long stretches of nucleotides is facilitated by DNA bending. Functional specificity as well as diversity are feasible with a limited number of transcription factors through alterations in the architecture of interaction between a group of proteins bound to promoter elements

Role of Citrate Ions in the Phosphonate-based Inhibitor System for Mild Steel in Aqueous Chloride Media

The corrosion inhibition efficiency of phosphonic acid and its derivatives for the inhibition of corrosion of mild steel in neutral chloride media is decided by its ability to form protectivefilm over the surface. In this context, the effect of addition of metal cations and certain organic compounds, such as citrate in conjugation with phosphonic acid to impart synergistic corrosion inhibition has been explored. The experiments were carried out using various concentrations of trisodium citrate and zinc ions in an aqueous solution of 2-carboxyethyl phosphonic acid (2CEPA) at 25 ppm. The corrosion characteristics have been determined using electrochemical impedance spectroscopy together with determination of corrosion rate by weight-loss method. It has been observed that a combination of inhibitive ions, namely citrate, ZCEPA, and zinc ions at 25 ppm gives 96 per cent inhibition efficiency and this corrosion inhibition is due to the formation of a protective film. By increasing the concentration of citrate beyond 25 ppm, the corrosion inhibition efficiency decreases. This paper discusses the role of citrate and zinc ions in imparting added corrosion inhibition ability using 2CEPA on the basis of experimental results

Solvable Map Representation Of A Nonlinear Symplectic Map

The evolution of a particle under the action of a beam transport system can be represented by a nonlinear symplectic map M. This map can be factorized into a product of Lie transformations. The evaluation of any given lie transformation in general requires the summation of an infinite number of terms. There are several ways of dealing with this difficulty: The summation can be truncated, thus producing a map that is nonsymplectic, but still useful for short term tracking. Alternatively, for long term tracking, the Lie transformation can be replaced by some symplectic map that agrees with it to some order and can be evaluated exactly. This paper shows how this may be done using solvable symplectic maps. A solvable map gives rise to a power series that either terminates or can be summed explicitly. This method appears to work quite well in the various examples that we have considered

Amplitude death in complex networks induced by environment

We present a mechanism for amplitude death in coupled nonlinear dynamical systems on a complex network having interactions with a common environment-like external system. We develop a general stability analysis that is valid for any network topology and obtain the threshold values of coupling constants for the onset of amplitude death. An important outcome of our study is a universal relation between the critical coupling strength and the largest non-zero eigenvalue of the coupling matrix. Our results are fully supported by the detailed numerical analysis for different network topologies.Comment: 7 pages, 7 figure

Drugs and drug targets against malaria

The development of resistance by the parasite against first line and second line antimalarial drugs, has underscored the importance to develop new drug targets and pharmacophores to treat the disease. The absence of a vaccine for protection and the availability of artemisinin and its derivatives as the only option has made the situation rather serious. With the availability of increased support for malaria research, a variety of drug targets and candidate molecules are now available for further development. However, the success rate of a candidate molecule to become a drug is very low and it does become necessary to start with a large basket, identified on a rational basis. This review focuses on the present efforts to identify a variety of drug targets in the malaria parasite and to develop candidate drug molecules

Making On-Demand Routing Efficient with Route-Request Aggregation

In theory, on-demand routing is very attractive for mobile ad hoc networks (MANET), because it induces signaling only for those destinations for which there is data traffic. However, in practice, the signaling overhead of existing on-demand routing protocols becomes excessive as the rate of topology changes increases due to mobility or other causes. We introduce the first on-demand routing approach that eliminates the main limitation of on-demand routing by aggregating route requests (RREQ) for the same destinations. The approach can be applied to any existing on-demand routing protocol, and we introduce the Ad-hoc Demand-Aggregated Routing with Adaptation (ADARA) as an example of how RREQ aggregation can be used. ADARA is compared to AODV and OLSR using discrete-event simulations, and the results show that aggregating RREQs can make on-demand routing more efficient than existing proactive or on-demand routing protocols

La Vida e historia del rey Apolonio [¿Zaragoza, Juan Hurus, 1488?] y su trayectoria genérica*

En el artículo se analiza la diversa trayectoria genérica de la Vida e historia del rey Apolonio [¿Zaragoza, Juan Hurus, 1488?] desde una triple perspectiva. Por su origen, remonta a la Historia Apollonii regis Tyri, narración de origen clásico próxima a las novelas griegas. Su recepción hispánica se aleja de su género de creación, pues el texto es traducción de un capítulo de las Gesta romanorum, y se presentaría, impreso junto a los Siete sabios de Roma, como una historia ejemplar. Por último, la crítica moderna ha optado por insertarlo en la serie de los «romances de materia clásica» y en el género editorial de las «historias caballerescas breves»